Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners

MSL975057 Mapping and Delivery Guide
Perform histological tests

Version 1.0
Issue Date: May 2024


Qualification -
Unit of Competency MSL975057 - Perform histological tests
Description
Employability Skills
Learning Outcomes and Application
Duration and Setting X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting.
Prerequisites/co-requisites
Competency Field
Development and validation strategy and guide for assessors and learners Student Learning Resources Handouts
Activities
Slides
PPT
Assessment 1 Assessment 2 Assessment 3 Assessment 4
Elements of Competency Performance Criteria              
Element: Receive and check specimens and associated request forms
  • Identify specimens and request forms that do not comply with workplace requirements
  • Record any discrepancies and indicate action required
  • Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee
  • Prepare work area for safe and effective workflows
  • Perform pre-use checks to ensure processor is fit for purpose
  • Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement
  • Record any issues that may impact on sample integrity and indicate action required
  • Check reagents are in sequential order
  • Select processor program for routine and non-routine samples
  • Perform pre-use check to ensure embedding centre is fit for purpose and uninterrupted embedding of processor load
  • Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy
  • Apply procedures to prevent cross-contamination between tissues
  • Inspect blocks, reject and re-embed items that do not meet quality control standards
  • Label required number of microscope slides in accordance with workplace traceability requirements
  • Check the flotation bath is ready and satisfactory for use
  • Prepare and adjust all external parts of a microtome and associated equipment for routine sample processing
  • Secure block in microtome following specified safety directions
  • Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts
  • Cut representative sections at the required thickness according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing
  • Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring patient identification on slides matches that on the block
  • Compare the blocks and sections ensuring the tissue on slides matches that in the blocks
  • Apply procedures to prevent cross-contamination between patient tissues
  • Identify, troubleshoot and resolve common section quality control issues
  • Inspect sections and reject any that do not meet quality control standards
  • Prepare any labile reagents for immediate use
  • Select reagents for specified technique, ensuring reagent sequence matches workplace procedure
  • Stain sections in accordance with workplace procedures using the required quality control section and accommodating any authorised variations
  • Mount slides using medium compatible with staining technique ensuring no bubbles
  • Examine control sections microscopically to ensure expected staining outcomes are achieved
  • Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain
  • Label specimens in accordance with workplace traceability requirements
  • Collate, approve and release slides to pathologist in accordance with workplace procedures
       
Element: Maintain laboratory records
  • Complete control stain quality assessment forms to report quality control outcome
  • File and store samples to facilitate efficient retrieval as required
  • Maintain instrument logs as required by accreditation checks
       

Submission Requirements

List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here

Assessment task 1: [title]      Due date:

(add new lines for each of the assessment tasks)


Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Identify specimens and request forms that do not comply with workplace requirements 
Record any discrepancies and indicate action required 
Register samples in accordance with workplace procedures, recording details that allow accurate tracking and chain of custody traceable to employee 
Prepare work area for safe and effective workflows 
Perform pre-use checks to ensure processor is fit for purpose 
Inspect processor reagents for deterioration and adequate volume, and report any items requiring replacement 
Record any issues that may impact on sample integrity and indicate action required 
Check reagents are in sequential order 
Select processor program for routine and non-routine samples 
Perform pre-use check to ensure embedding centre is fit for purpose and uninterrupted embedding of processor load 
Embed tissue types in correct orientation and characteristics that minimise tissue loss and artefacts during microtomy 
Apply procedures to prevent cross-contamination between tissues 
Inspect blocks, reject and re-embed items that do not meet quality control standards 
Label required number of microscope slides in accordance with workplace traceability requirements 
Check the flotation bath is ready and satisfactory for use 
Prepare and adjust all external parts of a microtome and associated equipment for routine sample processing 
Secure block in microtome following specified safety directions 
Orientate the block correctly for each specific tissue in order to maintain sample integrity and minimise artefacts 
Cut representative sections at the required thickness according to needs of subsequent procedures, maintaining sample integrity, minimising artefacts and conserving tissue for further testing 
Float sections onto water bath to flatten tissues and pick up sections onto microscope slides ensuring patient identification on slides matches that on the block 
Compare the blocks and sections ensuring the tissue on slides matches that in the blocks 
Apply procedures to prevent cross-contamination between patient tissues 
Identify, troubleshoot and resolve common section quality control issues 
Inspect sections and reject any that do not meet quality control standards 
Prepare any labile reagents for immediate use 
Select reagents for specified technique, ensuring reagent sequence matches workplace procedure 
Stain sections in accordance with workplace procedures using the required quality control section and accommodating any authorised variations 
Mount slides using medium compatible with staining technique ensuring no bubbles 
Examine control sections microscopically to ensure expected staining outcomes are achieved 
Confirm microscopically that the type/disease is appropriate and still present in the control sections for each stain 
Label specimens in accordance with workplace traceability requirements 
Collate, approve and release slides to pathologist in accordance with workplace procedures 
Complete control stain quality assessment forms to report quality control outcome 
File and store samples to facilitate efficient retrieval as required 
Maintain instrument logs as required by accreditation checks 

Forms

Assessment Cover Sheet

MSL975057 - Perform histological tests
Assessment task 1: [title]

Student name:

Student ID:

I declare that the assessment tasks submitted for this unit are my own work.

Student signature:

Result: Competent Not yet competent

Feedback to student

 

 

 

 

 

 

 

 

Assessor name:

Signature:

Date:


Assessment Record Sheet

MSL975057 - Perform histological tests

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

(add lines for each task)

Feedback to student:

 

 

 

 

 

 

 

 

Overall assessment result: Competent Not yet competent

Assessor name:

Signature:

Date:

Student signature:

Date: